Lab technician position: Circular RNAs in Alzheimer’s disease

Job Title
Lab technician position: Circular RNAs in Alzheimer’s disease


Requisition Number
DU12381


Department Name
7H180:Molecular and Cellular Biochemistry


Work Location
Lexington, KY


Salary Range
$16.30 to 18.00/hr.


Type of Position
Temporary


Position Time Status
Full-Time


Required Education

Bachelor’s degree or should be in a program to obtain a Bachelor’s Degree



Required Related Experience

n/a



Required License/Registration/Certification

n/a



Physical Requirements

Occasional repetitive motion, sitting and/or standing for long periods of time



Shift

The working hours are flexible after a training period. Should work full time (i.e. 35-40 hrs. per week).



Job Summary

A paid lab-tech position in Molecular Biology is available for a student or recent graduate with a strong desire to learn molecular biological techniques and to do research in the Stamm lab. The student should have at least a Bachelor’s degree or should be in a program to obtain a Bachelor’s Degree. We are looking for a person working full time for at least one year. Prior experimental experience is a plus. The funding for this project is secured for three years.



The project is centered on circular RNAs generated from the human tau locus that could be involved in Alzheimer’s disease (see Welden et al. 2018, PMID: 29729314).



Please also visit our website www.stamms-lab.net



The work involves modern molecular biology techniques, such as Gibson cloning, RT-PCR, Western Blots, cell culture and also purification of circRNA encoded proteins from brain. In addition, lab organization (media, ordering) should be performed.



The working hours are flexible after a training period. The person should work full time (i.e. 35-40 hrs. per week).



The anticipated stay is at least for one year.



Previous undergrad students (underlined) got their names on papers, which can be used for future grad/med school applications:



Arizaca Maquera KA, Welden JR, Margvelani G, Miranda Sardón SC, Hart S, Robil N, Hernandez AG, de la Grange P, Nelson PT, Stamm S. Alzheimer’s disease pathogenetic progression is associated with changes in regulated retained introns and editing of circular RNAs. Front Mol Neurosci. 2023 May 5;16:1141079. doi: 10.3389/fnmol.2023.1141079. PMID: 37266374



Welden JR, Margvelani G, Arizaca Maquera KA, Gudlavalleti B, Miranda Sardón SC, Campos AR, Robil N, Lee DC, Hernandez AG, Wang WX, Di J, de la Grange P, Nelson PT, Stamm S. RNA editing of microtubule-associated protein tau circular RNAs promotes their translation and tau tangle formation. Nucleic Acids Res. 2022 Dec 9;50(22):12979-12996. doi: 10.1093/nar/gkac1129. PMID: 36533443.



Welden JR, van Doorn J, Nelson PT, Stamm S. The human MAPT locus generates circular RNAs. Biochim Biophys Acta. 2018.PubMed PMID: 29729314.



Please send a short (2 page max) CV and a statement of interest (specific request 1) to

Stefan Stamm, Stefan@stamms-lab.net

And Giorgi Margvelani, margvelani@uky.edu and also apply to this posting.



Skills / Knowledge / Abilities

Does this position have supervisory responsibilities?
No


Preferred Education/Experience

Prior experimental experience is a plus



Deadline to Apply
10/31/2024


University Community of Inclusion


The University of Kentucky is committed to a diverse and inclusive workforce by ensuring all our students, faculty, and staff work in an environment of openness and acceptance. We strive to foster a community where people of all backgrounds, identities, and perspectives can feel secure and welcome. We also value the well-being of each of our employees and are dedicated to creating a healthy place to work, learn and live. In the interest of maintaining a safe and healthy environment for our students, employees, patients and visitors the University of Kentucky is a Tobacco & Drug Free campus.



As an Equal Opportunity Employer, we strongly encourage veterans, individuals with disabilities, women, and all minorities to consider our employment opportunities.




Any candidate offered a position may be required to pass pre-employment screenings as mandated by University of Kentucky Human Resources. These screenings may include a national background check and/or drug screen.